m1 primary polyclonal igg rabbit anti mouse antibodies for inos  (Danaher Inc)

Journal: Italian Journal of Animal Science

Article Title: Protective effect of chitosan oligosaccharide against oxidative damage of peripheral blood mononuclear cells in dairy cows induced by diethylenetriamine/nitric oxide via NF-κB signalling pathway

doi: 10.1080/1828051x.2020.1772131

Figure Lengend Snippet: Figure 2. Effects of chitosan oligosaccharideon on the phosphorylation level of NF-kB pathway, the protein expression level of nitric oxide synthase (iNOS) and interleukin-1b (IL-1b). Expressions of IL-1b(A); phosphorylated IjB kinase b (P-IKKb) (B); phosphorylated inhibitor of nuclear factor kappa-Ba (P-IjBa) (C); phosphorylated nuclear factor kappa-Bp65(P-NF-jBp65) (D); iNOS (E), protein levels were detected by western blotting and normalised to beta-actin (b-Actin) levels. CTR¼ control treatment, without chitosan oligosacchar- ides addition; COS40, COS80, COS160, and COS320¼ treated with 40, 80, 160, and 320lg/mL chitosan oligosaccharides respectively.

Article Snippet: The membranes were incubated in the following primary antibodies: primary polyclonal rabbit anti-iNOS antibody (NBP1-97471, Novus Biological, USA), rabbit anti-IL-1b (Cell Signalling Technology, Boston, MA), anti-IKKb, monoclonal rat anti-IjBa, monoclonal rabbit anti-NF-jBp65 antibody(NF-jB pathway Sampler Kit 9963,CST, MA), anti-phospho-IKKb, anti-phospho-IjBa, and anti-phospho-NF-jBp65 (NF-jB pathway Sampler Kit 9963, CST, MA).

Techniques: Phospho-proteomics, Expressing, Western Blot, Control